U.S. Next-generation Sequencing Library Preparation Market To Reach $1.96 Billion By 2033

U.S. Next-generation Sequencing Library Preparation Market Growth & Trends

The U.S. next-generation sequencing library preparation market size is expected to reach USD 1.96 billion by 2033, growing at a 13.09% CAGR from 2025 to 2033, according to a new report by Grand View Research, Inc. The market is experiencing strong growth driven by the increasing adoption of Next-generation Sequencing (NGS) technologies in clinical diagnostics, oncology, and personalized medicine. As healthcare systems and laboratories shift toward more precise and data-driven approaches, the demand for accurate and efficient library preparation solutions is rising. NGS enables the detection of genetic mutations, structural variations, and epigenetic changes, making it a powerful tool for diagnosing rare diseases, identifying cancer biomarkers, and tailoring treatments to individual patients. This clinical application surge is significantly boosting the need for high-quality library prep kits that can handle diverse and sensitive sample types.

In addition, government initiatives and research funding in genomics are propelling growth within academic and institutional sectors. U.S.-based research organizations and universities are investing in large-scale genomics projects, including whole genome, exome, and transcriptome sequencing. These projects require advanced library preparation methods to ensure consistency, throughput, and data integrity. As a result, manufacturers are focusing on developing automated, scalable, and cost-effective library prep kits to support both large-volume and specialized research workflows, further strengthening market demand.

The growing use of NGS in pharmaceutical and biotechnology companies for drug discovery, biomarker identification, and companion diagnostics is also a key market driver. These companies rely on sequencing data to accelerate clinical trials and enhance the precision of therapeutics. Library preparation is a critical component of this process, ensuring that sequencing libraries are uniform, reproducible, and compatible with high-throughput platforms. As precision medicine and targeted therapies continue to gain momentum, the need for robust, high-throughput library prep solutions will remain central to expanding the U.S. next-generation sequencing library preparation industry.

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U.S. Next-generation Sequencing Library Preparation Market Report Highlights

• By product, the reagents and consumables segment dominated the market with the largest share in 2024. This growth is driven by the increasing need for high-quality and dependable reagents for sample preparation, the broader adoption of NGS in both genomic research and clinical diagnostics.

• By application, the disease diagnostics segment is anticipated to grow at a significant CAGR during the forecast period. The growing prevalence of genetic and chronic diseases is driving demand for NGS-based diagnostic tools in the U.S. market. Library preparation plays a critical role in enabling accurate, high-throughput sequencing for early and precise disease detection.

• By end use, the pharmaceutical and biotechnology companies segment is anticipated to grow at the fastest CAGR of 14.51% during the forecast period. Pharmaceutical and biotechnology companies increasingly rely on NGS for drug development, biomarker discovery, and companion diagnostics. This drives demand for efficient, scalable library preparation solutions that ensure high data quality and regulatory compliance.

U.S. Next-generation Sequencing Library Preparation Market Segmentation:

Grand View Research has segmented the U.S. next-generation sequencing library preparation market report based on sequencing type, product, application, and end use:

U.S. Next-generation Sequencing Library Preparation Sequencing Type Outlook (Revenue, USD Million, 2021 - 2033)

• Targeted Genome Sequencing

• Whole Genome Sequencing

• Whole Exome Sequencing

• Other Sequencing Types

U.S. Next-generation Sequencing Library Preparation Product Outlook (Revenue, USD Million, 2021 - 2033)

• Reagents & Consumables

• DNA Library Preparation Kits

• RNA Library Preparation Kits

• Other Reagents & Consumables

• Instruments

U.S. Next-generation Sequencing Library Preparation Application Outlook (Revenue, USD Million, 2021 - 2033)

• Drug & Biomarker Discovery

• Disease Diagnostics

• Cancer Diagnostics

• Reproductive Health Diagnostics

• Infectious Disease Diagnostics

• Other Disease Diagnostic Applications

• Others

U.S. Next-generation Sequencing Library Preparation End Use Outlook (Revenue, USD Million, 2021 - 2033)

• Hospitals and Clinics

• Academic and Research Institutions

• Pharmaceutical and Biotechnology Companies

• Others

List of Key Players in the U.S. Next-generation Sequencing Library Preparation Market

• Illumina, Inc.

• Agilent Technologies, Inc.

• F. Hoffmann-La Roche AG, Inc.

• BD

• Oxford Nanopore Technologies

• New England Biolabs Inc.

• Revvity, Inc.

• QIAGEN N.V.

• Pacific Biosciences of California, Inc.

• Thermo Fisher Scientific Inc.