GVR Report cover In-vitro Toxicology Assays Market For Cannabis And Nicotine Testing Size, Share & Trends Report

In-vitro Toxicology Assays Market For Cannabis And Nicotine Testing Size, Share & Trends Analysis Report By Test Type, By Technology, By Application (Cytotoxicity Testing, Genetic Toxicity Testing), By Method, By Region, And Segment Forecasts, 2021 - 2028

  • Report ID: GVR-4-68039-551-1
  • Number of Pages: 117
  • Format: Electronic (PDF)
  • Historical Range: 2017 - 2019
  • Industry: Healthcare

Report Overview 

The in-vitro toxicology assays market size for cannabis and nicotine testing was valued at USD 1,236.1 million in 2020 and is expected to expand at a compound annual growth rate (CAGR) of 11.2% from 2021 to 2028. Rising usage of products based on cannabis and nicotine and the introduction of technologically advanced products are factors driving the in-vitro toxicology assay market for cannabis and nicotine testing.

Canada in-vitro toxicology assays market size for cannabis and nicotine testing, by application, 2018 - 2028 (USD Million)

In addition, the rise in an adolescent population, which is more prone to use nicotine and cannabis-related products, will aid in market growth. Furthermore, the number of countries legalizing the use of medical cannabis is expected to increase in the coming years resulting in increasing usage of medical cannabis across the globe. The potential for significant tax revenues from cannabis is another factor that has resulted in an increasing number of counties legalizing its medical use.

In the past few years, awareness regarding medical applications of cannabis (marijuana), such as appetite enhancement, pain management, and reducing eye pressure, is increasing. Owing to this factor, the number of countries legalizing the use of medical cannabis in various parts of this world is continuously increasing. For instance, most U.S. states have legally approved the use and commercialization of medical cannabis and an increasing number of countries are legalizing the plant for recreational use.

The increasing incidence of nicotine consumption-associated diseases has triggered the launch of various policies and initiatives by governments and global organizations to increase awareness among people. For instance, the CDC has initiated a project for reducing the consumption of sodium and tobacco to prevent COPD and cardiac disorders in China.

In recent years, the use of cannabis products has expanded, leading to an increasing number of specialized cannabis testing laboratories. This intensified the market completion. For instance, with rigorous legislative requirements and high sample throughput, Laboratory Information Management Systems (LIMS) serve as a promising tool for a testing laboratory.

Test Type Insights

The nicotine testing segment dominated the market and accounted for the largest revenue share of 54.0% in 2020. This can be attributed to the presence of an effective regulatory structure for the safe consumption of nicotine-containing products. Rising consumption of tobacco and the growing focus of research studies on nicotine testing utilization are factors expected to expedite segment growth.

The cannabis testing segment also captured a significant market share as it is used for detecting the quantity of cannabis substances in samples. This testing aids in monitoring regulated cannabis products in the market. Rising acceptance of cannabis for medical use is further anticipated to boost the market growth throughout the forecast period.

Technological advancements in cannabis testing are driving the segment growth. For instance, a test developed by PathogenDx, QuantX assay, which is intended for cannabis and hemp, measures the total amount of yeast and mold in a sample, while also assessing whether it meets industry testing as well as safety standards. The test takes less than 6 hours to complete. QuantX is ideal for cannabis testing labs as well as growers whose product must withstand a certain level of yeast and mold contamination.

Technology Insights

The mass spectrometry segment dominated the market and accounted for the largest revenue share of 25.9% in 2020. Increasing inspection by regulatory bodies will boost segment growth, as it assists in identifying unknown substances and quantify known substances. The rising focus of research studies on evaluating applications of mass spectrometry is aiding segment growth.

For instance, a research study published in the Journal of the American Society for Mass Spectrometry shared the current significance of mass spectrometry in the cannabis market. Experts emphasize on use of GC/MS and LC/MS/MS techniques in profiling terpenes and the detection of heavy metals, in addition to potency analysis and pesticide testing. This study also reports a few areas where mass spectrometry could be implemented or expanded.

The 3D cell culture technology segment is anticipated to grow at a rapid pace during the forecast period owing to the growing number of in-vitro toxicology laboratories in key regions and the increasing number of tests being conducted by them. Technological advancements in 3D cell culture technology to expand its application across various research studies are also driving the segment.

Application Insights

The cytotoxicity testing segment dominated the market and accounted for the largest revenue share of 27.2% in 2020. The segment is expected to continue its dominance over the forecast period. The rising significance of these testing methods in various technologies is considered to be a driving factor for market growth.

For instance, 3D cell cytotoxicity evaluation system technology can be used to assess cigarette smoke; this system offers faster results without any intervention of skilled force. Moreover, advancements in different tools, such as real-time cell analysis tool, which allows cytotoxic assessment of test substances, and the potential of such tools for evaluating the cytotoxic effects of nicotine-containing products is driving the segment growth.

The application of carcinogenicity testing for tobacco products has increased owing to the detection of chemicals containing carcinogenic properties. Thus, the evaluation of carcinogenic potential in these products is likely to enhance the adoption of such testing methods.  The in-vitro Cell Transformation Assays (CTA) is generally used to test chemicals and complex mixtures for their carcinogenic potential. This assay is responsible for detecting both genotoxic and non-genotoxic carcinogens.

Method Insights

The cellular assay testing method segment dominated the market and accounted for the largest revenue share of 58.8% in 2020 due to the rise in drug discovery screening to quantify cytotoxicity and other bio-chemicals. The launch of new products for cannabis-based medicine testing is further driving the market.

Advancements in cell-based assays are anticipated to positively impact revenue generation of the segment, owing to delivery of rapid and accurate results without any intervention of skill-force. For instance, Real-time Cell Analysis (RTCA) is a tool that enables rapid and relatively labor-free cytotoxic assessment of test substances. It is one such tool that has gained popularity as a result of its versatility, ease of use, and capacity to track cell state during an experiment.

Global in-vitro toxicology assays market for cannabis and nicotine share, by method, 2020 (%)

Other testing methods such as microbiological testing, including biochemical-based tests and molecular tests, for cannabis are performed in various laboratories. Considering the rising number of cannabis or nicotine-based products and the utilization of these products, the need for microbial detection has increased.

Regional Insights

North America dominated the in-vitro toxicology assays market for cannabis and nicotine testing and accounted for the largest revenue share of 40.4% in 2020. This growth in the region can be attributed to the presence of a substantial number of market players and legalized usage of cannabis and nicotine for medicinal and recreational purposes.

High healthcare expenditure, well-established healthcare infrastructure, and stringent regulations by FDA and Health Canada are some other factors contributing to the dominance of the region throughout the forecast period. The presence of research institutions and projects backed by Health Canada is likely to boost the market by a small margin.

In Asia Pacific, the market is likely to witness the fastest growth over the forecast period. The legalization of cannabis and nicotine-related products is expected to be the key market driver over the forecast period. It is anticipated that India will be the fastest-growing market for in-vitro toxicology assays in the region. CBD and other cannabis-related products are picking up the pace in Indian industries, with 4 native CBD brands in India.

Key Companies & Market Share Insights

Advancements in products, regional expansions, and strategic initiatives are key strategies that can aid in market development. The operating companies are undertaking extensive strategic initiatives in the field of in-vitro toxicology assay in cannabis and nicotine testing, including mergers and acquisitions, new product development, and regional expansion, to address the unmet needs of consumers.

For instance, in July 2021, IONTOX announced a collaboration with cbdMD, Inc. to identify novel cannabinoids for therapeutic use. The company is expected to perform the required toxicological studies with cbdMD Therapeutics. Some of the prominent players in the in-vitro toxicology assays market for cannabis and nicotine testing include:

  • Stemina Biomarker Discovery, Inc.

  • Broughton Nicotine Services

  • Labstat International, Inc.



  • Integrated Laboratory Systems

  • Enthalpy Analytical

  • PBR Laboratories, Inc.

In-vitro Toxicology Assays Market For Cannabis And Nicotine Testing Report Scope

Report Attribute


Market size value in 2021

USD 1,359.7 million

Revenue forecast in 2028

USD 2.9 billion

Growth rate

CAGR of 11.2% from2021 to 2028

Base year for estimation


Historical data

2017 - 2019

Forecast period

2021 - 2028

Quantitative units

Revenue in USD million and CAGR from 2021 to 2028

Report coverage

Revenue forecast, company ranking, competitive landscape, growth factors, and trends

Segments covered

Test type, technology, application, method, region

Regional scope

North America; Europe; Asia Pacific; Latin America; Middle East & Africa

Country scope

U.S.; Canada; Germany; U.K.; France, Italy, Spain, Japan; China; India; Australia, Singapore, Brazil; Mexico; Argentina; South Africa; Saudi Arabia

Key companies profiled

Stemina Biomarker Discovery, Inc.; Broughton Nicotine Services; Labstat International, Inc.; IONTOX; TOXIKON; Integrated Laboratory Systems; Enthalpy Analytical; PBR Laboratories, Inc.

Customization scope

Free report customization (equivalent up to 8 analyst’s working days) with purchase. Addition or alteration to country, regional, and segment scope

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Segments Covered in the Report

This report forecasts revenue growth at global, regional, and country levels and provides an analysis of the latest industry trends in each of the sub-segments from 2017 to 2028. For the purpose of this report, Grand View Research has segmented the global in-vitro toxicology assays market report for nicotine and cannabis testing on the basis of test type, technology, application, method, and region:

  • Test Type Outlook (Revenue, USD Million, 2017 - 2028)

    • Cannabis Testing

    • Nicotine Testing

  • Technology Outlook (Revenue, USD Million, 2017 - 2028)

    • 3D Cell Culture Technology

    • Mass Spectrometry

    • Flow Cytometry

    • Others

  • Application Outlook (Revenue, USD Million, 2017 - 2028)

    • Genetic Toxicity Testing

    • Carcinogenicity Testing

    • Cytotoxicity Testing

    • Mutagenicity Testing

    • Others

  • Method Outlook (Revenue, USD Million, 2017 - 2028)

    • Cellular Assay

      • Live Cells

        • High Throughput / High Content Screening

        • Molecular Imaging

        • Others

      • Fixed Cells

    • Others

  • Regional Outlook (Revenue, USD Million, 2017 - 2028)

    • North America

      • U.S.

      • Canada

    • Europe

      • Germany

      • U.K.

      • France

      • Spain

      • Italy

    • Asia Pacific

      • Japan

      • China

      • India

      • Australia

      • Singapore

    • Latin America

      • Brazil

      • Mexico

      • Argentina

    • Middle East & Africa

      • South Africa

      • Saudi Arabia

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