Rising number of genomic and proteomic studies which involve gene expression, SNP genotyping, protein extraction & purification is anticipated to drive cell lysis & dissociation market. Furthermore, increase in the focus on precision medicine, especially oncology research which involves the use of malignant growth is anticipated to drive market with lucrative avenues.
While in animals the cellular organelles are separated from the environment by a plasma membrane alone, in bacteria and plants the plasma membrane is additionally surrounded by a rigid cell wall. It consists of multiple cellulose layers in plants, while in bacteria it is composed of peptidoglycan. Thus, the lysis method is usually selected based on their source as the structure of membrane/wall varies significantly.
Conventionally, physical disruption methods were widely used owing to ease of implementation. However, the requirement for cumbersome and expensive equipment and availability of reagent-based methods which reduce the loss of valuable sample have limited usage of this method.
Modern laboratory research & diagnostic tests which require small volume and high-throughput processing make reagent-based method suitable for recent applications. Market players are providing various enzymes for dissociation & lysis based on their type and application such as cellulase, pectinase, & pectolyase are useful in plant protoplast preparation.
However, the shifting demand for efficient dissociation of tumor tissue into single cells has led to the development of novel products. Microfluidic devices are reported to significantly improve the recovery in terms of purity and number.
Various methods are investigated for effective whole genome amplification using microfluidics platforms. Rigid walls pose a major challenge in the bacterial whole genome amplification. Anticipated launch of novel products in the coming years is projected to influence progress for cell lysis & dissociation market.
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